HPVomics

A therapeutic and epitome resource for Human Papillomaviruses

Efficient putative sgRNAs (CRISPR/Cas9 targets)

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S.No.	HPV type	Target region	sgRNA seuences	PAM	Strand(F/R)	Start	End	GC (%)	Predicted effeciency (%)
1	HPV6	E8^E2	CCTTGTGTCCTCAAGCACCA	AGG	F	99	118	55	62.24
2	HPV6	E8^E2	GGTGCTTGAGGACACAAGGG	TGG	R	427	446	60	54.31
3	HPV6	E8^E2	CATATGATAGTGAGGAACAA	AGG	F	443	462	35	45.71
4	HPV6	E8^E2	CTTGGTGCTTGAGGACACAA	GGG	R	424	443	50	44.35
5	HPV6	E8^E2	GTATGTAGTAGATTCAGGAA	TGG	R	466	485	35	44.24
6	HPV6	E8^E2	GCTTGAGGACACAAGGGTGG	AGG	R	430	449	60	41.11
7	HPV6	E8^E2	TTGATTTAATATCATCAACG	TGG	F	371	390	25	40.19
8	HPV11	E8^E2	GGTGCAGGCGGACACTGTAG	GGG	R	421	440	65	62.19
9	HPV11	E8^E2	GTATGTAGTAGGTTCAGCAA	TGG	R	463	482	40	47.95
10	HPV11	E8^E2	TACAGTGTCCGCCTGCACCA	CGG	F	96	115	60	46.98
11	HPV11	E8^E2	CCACCATTAGGCATAAGGTG	GGG	F	488	507	50	43.79
12	HPV11	E8^E2	ACGCCTATAGTGCAACTGCA	AGG	F	280	299	50	41.95
13	HPV11	E8^E2	TGTGGCCAACATCAGATCCG	TGG	F	183	202	55	40.41
14	HPV40	E8^E2	GGGGGCGTCGGGGGTGTCGG	TGG	R	439	458	85	55.62
15	HPV40	E8^E2	GTAATTCCTCAACTACATGG	AGG	F	395	414	40	54.25
16	HPV40	E8^E2	GGTGTCGGTGGCAGTGGTCG	GGG	R	451	470	70	51.97
17	HPV40	E8^E2	GGCGTCAGCAGTCTCAACAA	TGG	R	478	497	55	51.54
18	HPV40	E8^E2	GGCGGGGCCGACCGTTTCGG	TGG	R	406	425	80	49.52
19	HPV40	E8^E2	CCTGACCCTGAAAGCAACAA	CGG	F	262	281	50	47.63
20	HPV40	E8^E2	ACAGCGACCACCGAAACGGT	CGG	F	124	143	60	44.97
21	HPV40	E8^E2	ACTGTGGGCAAATCACTCGG	AGG	F	205	224	55	44.53
22	HPV40	E8^E2	ACACCTATAATACAATTAGA	AGG	F	313	332	25	44
23	HPV40	E8^E2	GGGGTGTCGGTGGCAGTGGT	CGG	R	449	468	70	43.71
24	HPV40	E8^E2	GGGCACAGGAACTGTGGTGG	AGG	F	283	302	65	42.93
25	HPV42	E8^E2	GCGTAGCCTGACTTCCACAG	TGG	R	261	280	60	57.86
26	HPV42	E8^E2	ATACAGGATTTTATCCCAGA	AGG	R	32	51	35	46.99
27	HPV43	E8^E2	GGTGCACGTGGAATCTGGGA	CGG	R	433	452	60	58
28	HPV43	E8^E2	GGTTGTGCCCTCTAGGGTGG	TGG	R	264	283	65	54.21
29	HPV43	E8^E2	CACCCTAGAGGGCACAACCG	TGG	F	268	287	65	50
30	HPV43	E8^E2	CATATATTAGTGAACAACAG	AGG	F	455	474	30	46.99
31	HPV43	E8^E2	TTGCTGATGTGTCATCCACA	TGG	F	380	399	45	46.96
32	HPV43	E8^E2	AGATTCAGTAGACGCAACAG	AGG	R	475	494	45	43.05
33	HPV43	E8^E2	GCGTCTACTGAATCTGACAC	CGG	F	61	80	50	42.67
34	HPV43	E8^E2	ACATCATTGTTAATGCTCCC	AGG	R	3	22	40	41.32
35	HPV44	E8^E2	CCGCCATGTAAACGACCCCG	AGG	F	154	173	65	62.31
36	HPV44	E8^E2	GGTGGTGGTGGTGGAGGAGG	AGG	R	469	488	70	61.72
37	HPV44	E8^E2	GGATGAGGCCTGTGCAAGGG	TGG	R	451	470	65	58.85
38	HPV44	E8^E2	ACTGCCCATTGGCACCGCCG	AGG	F	117	136	70	51.37
39	HPV44	E8^E2	TGTCAGAAACAGCGACTCCG	TGG	F	216	235	55	47.81
40	HPV44	E8^E2	GCCTCATCCACACTGCCCAT	TGG	F	106	125	60	47.12
41	HPV44	E8^E2	GGCGTTGTTGGTGTTTGTGG	GGG	R	364	383	55	45.08
42	HPV44	E8^E2	GGCCTGTGCAAGGGTGGTGG	TGG	R	457	476	70	43.56
43	HPV44	E8^E2	TGGCACCGCCGAGGACTGCG	TGG	F	126	145	75	41.36
44	HPV54	E8^E2	GGAGTTGTCGGTACGTGGTG	TGG	R	412	431	60	66.64
45	HPV54	E8^E2	ACACAGCACCTCAGACCCCG	TGG	F	186	205	65	60.12
46	HPV54	E8^E2	AATGACATAGATATACTAGG	TGG	R	20	39	30	53.84
47	HPV54	E8^E2	CCTATAGTAGTGTAGAACAA	AGG	F	437	456	35	49.31
48	HPV54	E8^E2	CTAGCTCGTTTTCTAGGCGG	CGG	R	368	387	55	48.47
49	HPV61	E8^E2	GGGTGGACAGTACTGACCAG	TGG	F	245	264	60	70.14
50	HPV61	E8^E2	GGGGGTGGTGGTGGTCTGTG	TGG	R	448	467	70	58.23
51	HPV61	E8^E2	AATCGTGTAACCATTCCAAA	GGG	F	511	530	35	53.94
52	HPV61	E8^E2	GGGTGGGGCCCACGCACGTG	GGG	R	429	448	80	53.11
53	HPV61	E8^E2	GGTGGGGCCCACGCACGTGG	GGG	R	430	449	80	51.46
54	HPV61	E8^E2	GGGGCCCACGCACGTGGGGG	TGG	R	433	452	85	47.19
55	HPV61	E8^E2	TTGACAAAGCATCATCCACA	TGG	F	404	423	40	41.4
56	HPV61	E8^E2	GCAGTGGCTTGTATACCCTT	TGG	R	26	45	50	41.03
57	HPV70	E8^E2	GTAAAGGCTGCAACAAACGA	CGG	F	212	231	45	49.91
58	HPV70	E8^E2	GGAAGTGGAAACAGCTGAAG	TGG	F	11	30	50	49.38
59	HPV70	E8^E2	GGTGCATGGGGTTGTTGCGG	CGG	R	373	392	65	41.48
60	HPV70	E8^E2	GGCAAGGGAAGTAAACATAC	AGG	F	370	389	45	40.81
61	HPV70	E8^E2	ATATTTCATGTACTTGGCAT	TGG	F	341	360	30	40.36
62	HPV70	E8^E2	TGTTGCGGCGGTATGGGCCG	GGG	R	385	404	70	40.12
63	HPV72	E8^E2	GGCGTGACAGTGCTGACCAG	TGG	F	248	267	65	56.48
64	HPV72	E8^E2	GGTATGTGGATACTGACCAA	CGG	F	482	501	45	51.99
65	HPV72	E8^E2	AAGTCGTGTGAACATTCCAA	AGG	F	516	535	40	47.29
66	HPV72	E8^E2	GGGGCCCACGCACGTGGCGG	TGG	R	442	461	85	47.26
67	HPV72	E8^E2	AATGTGCATTTGTAACACTG	TGG	F	461	480	35	44.22
68	HPV72	E8^E2	AGTCGTGTGAACATTCCAAA	GGG	F	517	536	40	42.86
69	HPV72	E8^E2	GCACCTATAATACATTTAAA	AGG	F	328	347	25	41.09
70	HPV81	E8^E2	GCACCTGTAATACATTTGCG	AGG	F	316	335	45	72.81
71	HPV81	E8^E2	GGGCCCCGCCAAGACCACCA	AGG	F	132	151	75	62.19
72	HPV81	E8^E2	GGTGGACAATAACAAATCAG	GGG	F	240	259	40	62.05
73	HPV81	E8^E2	GGTACTGGTGGCGGGTTCGG	GGG	R	445	464	70	53.39
74	HPV81	E8^E2	ACAACGTGTGACAATACCAA	AGG	F	504	523	40	51.03
75	HPV81	E8^E2	GGGGCCTTGGTGGTCTTGGC	GGG	R	410	429	70	50.02
76	HPV81	E8^E2	GGGCCTTGGTGGTCTTGGCG	GGG	R	411	430	70	49.45
77	HPV81	E8^E2	GGGGCCCACGTGCTGGGTAC	TGG	R	430	449	75	45.48
78	HPV81	E8^E2	TGCACCACCGAACATCTGGG	TGG	F	222	241	60	44.83
79	HPV81	E8^E2	CAACGTGTGACAATACCAAA	GGG	F	505	524	40	44.49
80	HPV81	E8^E2	ACAGGTCCAATGCCATGTGG	AGG	R	136	155	55	43.3
81	HPV81	E8^E2	TCCACAGGTCCAATGCCATG	TGG	R	133	152	55	41.59
82	HPV81	E8^E2	GGGTGGACAATAACAAATCA	GGG	F	239	258	40	40.85